Forensic Pocket Guide

Dye Stain

Safranin O

Common name: Basic Red 2

1. Select an application method.

Tray immersion: Submerge item in Safranin O solution.

Spray application: Evenly spray Safranin O solution with a squirt bottle or sprayer onto item. 

2. Allow item to air dry.

3. Ridges will develop a red color (yellow fluorescence). Item may be rinsed with methanol to remove any excess Safranin O solution. 

4. View ridge detail using a laser or forensic light source at 500nm using orange goggles. 

5. Capture ridge detail using 1:1 photography and an orange colored or 550 (BP 35) barrier filter.

View under

500nm

Goggles / Filter

Orange

Development Color

Yellow

REFERENCES

Chesapeake Bay Division International Association of Identification. (2009). “Safranin O”. Retrieved from https://www.cbdiai.org/safranin-o.html

FBI Laboratory Division. (2000). “Safranin O (Fluorescent Dye)”, Processing Guide for Developing Latent Prints, pp. 59. Retrieved from https://archives.fbi.gov/archives/about-us/lab/forensic-science-communications/fsc/jan2001/lpu.pdf