1. Select an application method.

Tray immersion: Submerge item in Nile Red solution.

Spray application: Evenly spray Nile Red solution with a squirt bottle onto the item.

2. Allow item to air dry.

3. Ridges will develop a reddish pink color (orange fluorescence) and can be captured using 1:1 photography.

4. View ridge detail using a laser or forensic light source in the 450 – 560nm using orange or red colored goggles.

4. Capture ridge detail using 1:1 photography and an orange or 550 (BP 35) bandpass barrier filter.