Forensic Pocket Guide

Dye Stain

Merbromin

DISCLAIMER: Area of suspected blood must be dry prior to the application of Merbromin. The application of Merbromin may also degrade DNA. 

1. Evenly spray item with Working Solution A (Merbromin Solution) using a fine-mist aerosol applicator. 

2. Allow item to air dry completely. 

3. Spray item a second time with Working Solution A (Merbromin Solution) and allow item to air dry completely again. 

4. Evenly spray item with Working Solution B (Hydrogen Peroxide Solution) using a fine-mist aerosol applicator. 

5. Allow item to air dry completely. 

6. Spray item a second time with Working Solution B (Hydrogen Peroxide Solution) and allow item to air dry completely again.

7. Ridges will develop a yellow fluorescent color and can be captured using 1:1 photography.

8. View ridge detail using a forensic light source in the 435 – 515nm range with orange goggles. 

9. Capture ridge detail with 1:1 photography using an orange barrier filter.

Flashlight Icon White - Freepik

View under

435 - 515nm

Safety Goggles Black

Goggles / Filter

Orange

Magnifying Glass with Fingerprint Black

Development Color

Yellow

FPG logo 5

PRO TIP: Merbromin is helpful for developing prints on dark backgrounds when other blood enhancement reagents would lack contrast. 

REFERENCES

(2018). Technical Procedure for Merbromin. Retrieved  from https://forensicresources.org/wp-content/uploads/2019/07/Merbromin-01-19-2018.pdf

DeHaan, J.D. et al. Chemical Enhancement of Fingerprints in Blood: An Evaluation of Methods, Effects on DNA< and Assessment of Chemical Hazards. Retrieved from http://www.latent-prints.com/cac_blood.htm