Forensic Pocket Guide

Dye Stain

D.F.O.

1. Select an application method.

Tray immersion: Submerge item in D.F.O. for 30 seconds.

Spray application: Evenly spray D.F.O. with a squirt bottle and leave on item for 30 seconds. 

2. Allow item to air dry inside a fume hood for 30 seconds.

3. Apply a second application of D.F.O. using one of the methods described above and allow item to air dry inside a fume hood again.

4. Heat item at 100°C in a heating chamber for 10 minutes.  

5. Ridges will develop a reddish-pink color and can be captured using 1:1 photography.

6. View ridge detail using a forensic light source in the 495 – 550nm range with orange or red googles.

7. Capture ridge detail using 1:1 photography and an orange colored or 550 (BP 35) bandpass filter.

Flashlight Icon White - Freepik

View under

495 - 550nm

Safety Goggles Black

Goggles / Filter

Orange / Red

Magnifying Glass with Fingerprint Black

Development Color

Reddish-pink

FPG logo 5

PRO TIP: A hair dryer, iron (dry), or heat press machine may be used if a heating chamber is not available. 

REFERENCES

Technical Procedure for DFO (1, 8-Diazafluoren-9-one). Office of Indigent Defense Services. (2012, September 17). Retrieved July 21, 2021 from http://www.ncids.com/forensic/labs/Latent/Technical/DFO.pdf.