DISCLAIMER: Area of suspected blood must be dry prior to the application of D.A.B. Cyanoacrylate fuming and other blood enhancement protein stains may also be detrimental to D.A.B. processing, and therefore must be completed after D.A.B. processing. 

1a. Submersion Method

Tray 1: Submerge item in fixer solution (Solution A) for 3 – 5  minutes. 

Tray 2: Submerge item in distilled water for 30 – 60 seconds. 

Tray 3: Submerge item in developer solution for 5 minutes (maximum). Item may be removed prior to 5 minutes if maximum development or contrast has been achieved. 

Tray 4: Submerge item in distilled water to stop the developer solution from overdeveloping the ridge detail. 

1b. Tissue Method

Bottle 1: Apply fixer solution (Solution A) with a squirt bottle onto a tissue that has been placed over the item. Keep the tissue wet for 3 – 5 minutes. 

Bottle 2: Remove the tissue, then squirt distilled water on the item for 30 – 60 seconds. 

Bottle 3: Apply developer solution with a squirt bottle onto a new tissue that has been placed over the item. Keep the tissue wet for 5 minutes (maximum). The tissue may be removed prior to 5 minutes if maximum development or contrast has been achieved. 

Bottle 2 (repeated): Remove the tissue, the squirt distilled water onto the item for 30 – 60 seconds. 

2. Allow item to air dry.

3. Ridges will develop a brown color and can be captured using 1:1 photography.