Forensic Pocket Guide

Dye Stain

Basic Yellow 40

1. Select an application method.

Tray immersion: Submerge item in Basic Yellow 40 reagent for 5 seconds.

Aerosol sprayer: Evenly apply Basic Yellow 40 dye stain to item with aerosol sprayer and leave for 5 seconds. 

2. Rinse item with water via squirt bottle or gently running stream for 10 seconds.

3. Ridges will develop a yellow color and can be captured using 1:1 photography. 

4. View ridge development using a UV lamp at 365nm with UV protection googles, or using a forensic light source in the 450 – 485nm range with yellow googles.

5. Capture ridge detail using 1:1 photography and a yellow colored or 515 (BP 35) bandpass filter.

Flashlight Icon White - Freepik

View under

365nm or

450 - 485nm

Safety Goggles Black

Goggles / Filter

Clear UV or Yellow

Magnifying Glass with Fingerprint Black

Development Color

Yellow

REFERENCES

Arrowhead Forensics. (2021). Basic Yellow 40: Product Description. Retrieved from https://www.arrowheadforensics.com/a-26501-basic-yellow-40.html

Chesapeake Bay Division, International Association for Identification. (2010). Latent Fingerprint Processing Techniques – Selection & Sequencing Guide. “Basic Yellow 40”. Retrieved from https://www.cbdiai.org/basic-yellow-40.html

Sirchie. (2011). Technical Information: Basic Yellow Fluorescent Enhancement Dye. Retrieved from https://www.sirchie.com/media/resourcecenter/item/l/v/lv507_ti02-77eng-rev5e_1.pdf